Journal: Cancer Biology & Therapy

Article Title: CPT1A mediates the succinylation of SP5 which activates transcription of PDPK1 to promote the viability and glycolysis of prostate cancer cells

doi: 10.1080/15384047.2024.2329372

Figure Lengend Snippet: CPT1A is highly expressed in PCa tissues and cell lines. (a) GEPIA was used to analyze the dysregulated expression of CPT1A in PCa. PRAD: prostate adenocarcinoma. T: tumor and N: normal. (b) The expression of CPT1A in PCa tissues and the normal tissues was detected by qPCR. (c) The expression of CPT1A in PCa cell lines and the prostate epithelial cell line (PNT2) was detected by qPCR. * p <.05 in (A). ** p < .01, *** p < .001 vs PNT2 group.

Article Snippet: HEK-293T cells, normal human epithelial prostate PNT2 cells and the PCa cell lines including DU145, LNCap, 22Rv1 and VCaP were purchased from ATCC (USA) and were maintained in Dulbecco’s modified eagle medium containing 10% fetal bovine serum and 1% penicillin/streptomycin (all from Thermo Fisher Scientific, USA).

Techniques: Expressing

Journal: Scientific Reports

Article Title: Dynamic interplay between sortilin and syndecan-1 contributes to prostate cancer progression

doi: 10.1038/s41598-023-40347-7

Figure Lengend Snippet: Sortilin (SORT) interacts with progranulin (PRGN) and LPL in PCa cells and PRGN is secreted by aggressive PC3 cells. ( a ) Representative confocal images showing co-labelling of cells with anti-PRGN (red) and anti-SORT (green) antibodies. Scale bars; 10 µm. ( b ) Quantification of colocalisation between PRGN and SORT. ( c ) Amount of PRGN in conditioned media (CM) and corresponding cell lysates with quantification of band densities and ratio of PRGN in CM to PRGN in cell lysates. ( d ) Endogenous SORT was immunoprecipitated (IP) with anti-SORT antibodies and PRGN was detected by Western blotting. Input; cell lysate. ( e ) Representative confocal images showing co-labelling of cells with anti-LPL (red) and anti-SORT (green) antibodies. Scale bars; 10 µm. ( f ) Quantification of colocalisation between LPL and SORT. ( g ) Amount of LPL in CM and corresponding cell lysates with quantification of band densities in cell lysates. In ( c ) and ( g ) PNT2 bands from the same membrane were shifted towards LNCaP and PC3 bands. Western blotting signal was normalised using total protein staining. ( h ) Endogenous SORT was immunoprecipitated (IP) with anti-SORT antibodies and LPL was detected by Western blotting. Input; cell lysate. Data are presented as mean ± SD, in ( b , f ) n = 10 representative ROIs, in ( c , g ) n = 3 (independent experiments), one-way ANOVA.

Article Snippet: Human prostate cell PNT2 (ECACC 95012613), LNCaP (clone FCG, ECACC 89110211) and PC3 (ECACC 90112714) were obtained from the European Collection of Authenticated Cell Cultures (ECACC) from CellBank Australia (Children's Medical Research Institute, Westmead, NSW, Australia).

Techniques: Immunoprecipitation, Western Blot, Membrane, Staining

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Schistosoma Egg Antigen Induces Oncogenic Alterations in Human Prostate Cells

doi: 10.1155/2018/4675380

Figure Lengend Snippet: schSEA depletes GSH levels in prostate (PNT2) cells. Statistical significance by Student's t -test; ∗ p ≤ 0.05 (serum-fed vs. serum-starved experiments) and # p ≤ 0.05 (non-schSEA treatment vs. schSEA-treated cells/NaNO 2 -treated cells). Results are representative of three independent experiments.

Article Snippet: Normal human prostate (PNT2) cells obtained from RIKEN BioResource Centre Cell Bank (Japan) were maintained in RPMI-1640 culture medium enriched with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin and incubated at 37°C in the presence of 5% CO 2.

Techniques:

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Schistosoma Egg Antigen Induces Oncogenic Alterations in Human Prostate Cells

doi: 10.1155/2018/4675380

Figure Lengend Snippet: Effect of SEA treatment on proliferation of PNT2 cells.

Article Snippet: Normal human prostate (PNT2) cells obtained from RIKEN BioResource Centre Cell Bank (Japan) were maintained in RPMI-1640 culture medium enriched with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin and incubated at 37°C in the presence of 5% CO 2.

Techniques:

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Schistosoma Egg Antigen Induces Oncogenic Alterations in Human Prostate Cells

doi: 10.1155/2018/4675380

Figure Lengend Snippet: schSEA induces proliferation of prostate (PNT2). Statistical significance by Student's t -test: ∗ p ≤ 0.05 (non-schSEA treatment vs. 12.5 μ g/ml schSEA-treated cells). Results are representative of three independent experiments.

Article Snippet: Normal human prostate (PNT2) cells obtained from RIKEN BioResource Centre Cell Bank (Japan) were maintained in RPMI-1640 culture medium enriched with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin and incubated at 37°C in the presence of 5% CO 2.

Techniques:

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Schistosoma Egg Antigen Induces Oncogenic Alterations in Human Prostate Cells

doi: 10.1155/2018/4675380

Figure Lengend Snippet: schSEA diminishes apoptosis in PNT2 cells. (a) (A) Negative control, (B) 6.25 μ g/ml schSEA, (C) 12.5 μ g/ml schSEA, and (D) serum-fed (positive control). Lower left quadrant: viable cells; lower right quadrant: early apoptotic cells; upper left quadrant: nuclear debris; upper right quadrant: late apoptotic cells. Data was acquired by Guava EasyCyte™ flow cytometer. (b) Comparison of apoptosis and cell viability among various experimental groups. Statistical significance by Student's t -test: ∗ p ≤ 0.05 (serum-fed vs. serum-starved/schSEA-treated groups). Results are representative of three independent experiments.

Article Snippet: Normal human prostate (PNT2) cells obtained from RIKEN BioResource Centre Cell Bank (Japan) were maintained in RPMI-1640 culture medium enriched with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin and incubated at 37°C in the presence of 5% CO 2.

Techniques: Negative Control, Positive Control, Flow Cytometry, Comparison

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Schistosoma Egg Antigen Induces Oncogenic Alterations in Human Prostate Cells

doi: 10.1155/2018/4675380

Figure Lengend Snippet: schSEA diminishes nuclei condensation in PNT2 cells: (a) negative control, (b) 6.25 μ g/ml schSEA, (c) 12.5 μ g/ml schSEA, and (d) serum-fed (positive control). Condensed and morphologically altered nuclei indicated by arrows. Cells were stained with Hoechst 33258 dye and nuclei visualized with fluorescence microscope (magnification 40x) (Olympus DP 72, Japan).

Article Snippet: Normal human prostate (PNT2) cells obtained from RIKEN BioResource Centre Cell Bank (Japan) were maintained in RPMI-1640 culture medium enriched with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin and incubated at 37°C in the presence of 5% CO 2.

Techniques: Negative Control, Positive Control, Staining, Fluorescence, Microscopy